Use Of Biotechnology Techniques In Breeding
KDC 634.8: 631.527
METHODS OF BYOTECHNOLOGY IN THE SELECTION OF THE BEST-MONIC CONTRIBUTIONS
I.I. Potapenco, Novosticassk
A method is proposed to obtain basic material for the segregation of seedless varieties of grapes using biotechnology techniques.
In the twenty-first century, the adverse impact of factors such as the evolving global energy crisis and climate change in the world is increasingly being taken into account, which may lead to increased stress levels affecting living organisms. In such circumstances, biological and biophysical research aimed at the selective improvement of crops, the stability of production and the reduction of its losses are even more important than in the past. It has become clear that the most reliable and cost-effective factor in improving yields and their stability is class. The dependence of increased production from class is steadily increasing.
The creation of productive varieties has been made possible with new biotechnological methods for improving plants (genic, chromosome, cellular engineering) combined with improved classical seclusion methods. This provides new opportunities for targeted changes in crops and their resilience, productivity and quality .
One aspect of cellular technology is the use of it in sedition, which facilitates and accelerates the traditional selective process of creating new varieties and varieties of plants. Existing methods of cultivation of isolated cells and tissues in vitro can be divided into two rupees.
The first group is auxiliary technologies that do not replace or serve the usual seltration. These include: in vitro fertilization (preventing gastro incompatibility), cultivation of seeds and immature hybrid horizons (removal of post-gasm incompatibility), acquisition of gaults by cultivating dusts and microspores, screaming of isolated cells, tissues and organs, cloning microrid reproduction.
The second group of methods leads to self-reliant, independent of traditional methods. ♪ ♪
The correlation analysis showed that the closest relationship exists between the embryonic potential of seeds and the amount of heat during the period of boutonization (g = 0, 69), as well as fertilization and fertile (g = 0, 66).
The hybrid fund is filled with new forms. Unnamed forms of vineyard were produced: VI-15-1-2 (Kishmish TFL x Korinca Russian) III-IV of the class of seedless with a berry mass of 1, 7 g, average mass of seeds 17 mg and seed from the family of Kismish CGL x Kismish unique - mass of berries 2, 3 g, semeni - 30 mg.
Embryoculture provides an opportunity to grow hybrid plants from incomplete foetus. However, the output of hybrid plants is small. Researchers therefore often have the task of multiplying and sustaining the plants. This is supported by clonal micro-dissemination. Hybrids are multiplied by activating the development of grazing kidneys (worms of sterile runs), adventive kidneys or regeneration of plants from callus tissue.
Seians we received from the crossroads of both single parents were vegetatively multiplied by clonal micro-dissemination. The increase in the population of these forms increases the probability of their retention and allows further study on agrobiological grounds with a view to obtaining high-level, non-family hybrids with enhanced environmental sustainability.